Fluorophore engineering: gentle and robust dyes for 4D imaging
Presented by Dr. Zhixing Chen
Abstract:
4D fluorescence imaging is jointly enabled by new microscopic methods and novel fluorescent markers that are bright, photostable, and biocompatible. Notably, phototoxicity has become a prevailing issue in the super-resolution era when boosted illumination is applied, compromising the physiological relevance of the recorded data. We advocate leveraging chemical approaches to tackle phototoxicity. By exploiting chemical motifs such as triplet state quenchers and biocompatible auxiliaries, we systematically upgrade the commonly used fluorescent markers toward alleviated phototoxicity. These gentle dyes can be directed to various cellular targets spanning mitochondria, DNA, cytoskeleton, insulin granule, and specific proteins, enabling time-lapse super-resolution imaging with minimal photodamage. For example, PK Mito Orange probe is a mitochondrial inner membrane stain that enables 30 frames of STED recording and multi-color imaging of mitochondrial components. PK Zinc dyes enable multiplexed imaging of insulin secretion in isolated islets. These biocompatible probes, with high specificity and gentle behavior under excitation light, promise to offer reliable spatial-temporal information in the era of 4D multiplexed nanoscopy.
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